Characterization of mutations and loss of heterozygosity of p53 and K-\u3ci\u3eras\u3c/i\u3e2 in pancreatic cancer cell lines by immobilized polymerase chain reaction

Background The identification of known mutations in a cell population is important for clinical applications and basic cancer research. In this work an immobilized form of the polymerase chain reaction, referred to as polony technology, was used to detect mutations as well as gene deletions, resulting in loss of heterozygosity (LOH), in cancer cell lines. Specifically, the mutational hotspots in p53, namely codons 175, 245, 248, 249, 273, and 282, and K-ras2, codons 12, 13 and 61, were genotyped in the pancreatic cell line, Panc-1. In addition LOH analysis was also performed for these same two genes in Panc-1 by quantifying the relative gene copy number of p53 and K-ras2. Results Using polony technology, Panc-1 was determined to possess only one copy of p53, which possessed a mutation in codon 273, and two copies of K-ras2, one wildtype and one with a mutation in codon 12. To further demonstrate the general approach of this method, polonies were also used to detect K-ras2 mutations in the pancreatic cell lines, AsPc-1 and CAPAN-1. Conclusions In conclusion, we have developed an assay that can detect mutations in hotspots of p53 and K-ras2 as well as diagnose LOH in these same genes

A revised and updated Odonata checklist of Samoa (Insecta: Odonata)

Odonata records of the Samoan Archipelago are updated and an updated checklist provided. It is part of an ongoing assessment of the fauna, taxonomy and distributionof the Pacific island dragonflies. The checklist follows recent reviews published/prepared about the Solomon Islands, New Caledonia, Fiji and Kingdom of Tonga. This study draws on recent dragonfly records following general insect surveys spanning 2008-2012 funded by Critical Ecosystem Partnership Fund (CEPF) via Conserva-tion International (CI) to the authors and to Secretariat Pacific Regional Environment Program (SPREP) and also by funding from Japan International Cooperation Agency (JICA). Other unpublished data from Samoan Archipelago and Niue are included as well. All, but one, of the newly collected Odonata species are widespread within the Pacific region. Hemicordulia cupricoloris the only species from the recent collections which is endemic to Samoa, previously reported for Savai'i and Upolu Islands. It has neverbeen confirmed since its original description in 1927. The new study shows the species as an inhabitant of high altitude zones of Savai'i. It is recommended inland areas of Savai'i and other islands within the Samoan Archipelago should be targeted in further field studies

Stewards of the Forest: An Analysis of Ginseng Harvesters and the Communal Boundaries That Define Their Identity in an Area of Environmental Degradation

Research introduced here demonstrates the use of protagonist framing as a means of identifying the boundaries that define a community’s identity in relation to an antagonist. Specifically, this research examines the two-sided nature of boundaries and the impact such boundaries have on the identity of a community. Through the telling narrative of two distinctively contrasting members of the ginseng steward community, this research explores how boundaries and protagonist framing can be used to identify the schemata of interpretation that enables the ginseng steward community to locate, perceive, and label themselves in relation to American society and a capitalist mentality. The author interprets the boundaries used by the ginseng steward community as a means of understanding for American society and as being reflective of the steward’s identity through their adherence to these boundaries as being central components of their identity. The research finds that the boundaries maintained by ginseng stewards influence and even dictate their notions of stewardship, sustainability, morality, and American society. Drawing on ten months of ethnographic research involving interviews and observations of the everyday activity conducted by members of the ginseng steward community, this research contributes to our understanding of how boundary identification can be used to classify and discuss a community’s identity and their perception of non-community members

Evolutionary Dynamics of Intron Size, Genome Size, and Physiological Correlates in Archosaurs

It has been proposed that intron and genome sizes in birds are reduced in comparison with mammals because of the metabolic demands of flight. To test this hypothesis, we examined the sizes of 14 introns in a nonflying relative of birds, the American alligator (Alligator mississippiensis), and in 19 flighted and flightless birds in 12 taxonomic orders. Our results indicate that a substantial fraction (66%) of the reduction in intron size as well as in genome size had already occurred in nonflying archosaurs. Using phylogenetically independent contrasts, we found that the proposed inverse correlation of genome size and basal metabolic rate (BMR) is significant among amniotes and archosaurs, whereas intron and genome size variation within birds showed no significant correlation with BMR. We show statistically that the distribution of genome sizes in birds and mammals is underdispersed compared with the Brownian motion model and consistent with strong stabilizing selection; that genome size differences between vertebrate clades are overdispersed and punctuational; and that evolution of BMR and avian intron size is consistent with Brownian motion. These results suggest that the contrast between genome size/BMR and intron size/BMR correlations may be a consequence of different intensities of selection for these traits and that we should not expect changes in intron size to be significantly associated with metabolically costly behaviors such as flight.Organismic and Evolutionary Biolog

Senior Recital: Nassar Edwards, violin

This recital is presented in partial fulfillment of requirements for the degree Bachelor of Music in Music Education. Mr. Edwards studies violin with Helen Kim.https://digitalcommons.kennesaw.edu/musicprograms/2152/thumbnail.jp

THE ROLE OF CONSERVATION RESEARCH AND EDUCATION CENTERS IN GROWING NATURE-BASED TOURISM

There are increasing numbers of private (nonprofit and for-profit) centers that carry out conservation research and education in locations of environmental concern. Such centers generate revenue streams that directly support conservation programs and also sustain surrounding human communities. This paper assesses the size of the centers’ economic impacts. We conducted separate studies of the economic impacts of the Cheetah Conservation Fund (Namibia) and (jointly) the Rowe Bird Sanctuary and Whooping Crane Trust (central Nebraska, USA). We collected data on direct expenditures and surveyed visitors and volunteers on their spending. For the Cheetah Conservation Fund, we estimate total economic impact using a Social Accounting Matrix developed for Namibia to determine appropriate multipliers. For the Rowe Sanctuary and the Whooping Crane Trust, we employ the IMPLAN Pro modeling software. We find that the Cheetah Conservation Fund generates a total economic impact of US$4.13 million per year and Rowe Sanctuary/Whooping Crane Trust generates US$3.80 million annually; the former sustains 177 jobs and the latter creates 63 jobs. Are such impacts significant? Two considerations suggest they are. First, such centers tend to be located in remote, usually rural areas where even small impacts may be important in sustaining local human communities. Second, for Africa alone we identified some 352 active conservation centers (undoubtedly a large undercount), so if on average each had an economic impact equal to that of Cheetah Conservation Fund, their combined impact would total about $1.5 billion per year

Non-equilibrium temperatures in steady-state systems with conserved energy

We study a class of non-equilibrium lattice models describing local redistributions of a globally conserved quantity, which is interpreted as an energy. A particular subclass can be solved exactly, allowing to define a statistical temperature T_{th} along the same lines as in the equilibrium microcanonical ensemble. We compute the response function and find that when the fluctuation-dissipation relation is linear, the slope T_{FD}^{-1} of this relation differs from the inverse temperature T_{th}^{-1}. We argue that T_{th} is physically more relevant than T_{FD}, since in the steady-state regime, it takes equal values in two subsystems of a large isolated system. Finally, a numerical renormalization group procedure suggests that all models within the class behave similarly at a coarse-grained level, leading to a new parameter which describes the deviation from equilibrium. Quantitative predictions concerning this parameter are obtained within a mean-field framework.Comment: 16 pages, 2 figures, submitted to Phys. Rev.

The Role of Compensation in Nicotine Reduction.

The available research on switching from normal nicotine to very low nicotine content cigarettes shows minimal evidence of compensatory smoking. Mathematical estimations suggest that substantial compensation after switching to very low nicotine cigarettes would be impossible. It is likely that smokers who are unable to tolerate the extent of proposed nicotine reduction would switch to other sources of nicotine, rather than try to compensate by smoking more very low nicotine content cigarettes more intensely